Journal: PLoS ONE

Article Title: Expression of Vesicular Glutamate Transporters VGLUT1 and VGLUT2 in the Rat Dental Pulp and Trigeminal Ganglion following Inflammation

doi: 10.1371/journal.pone.0109723

Figure Lengend Snippet: VGLUT1- and VGLUT2- immunopositive axons costained for PGP9.5 (arrowheads) indicating VGLUT1 and VGLUT2 were expressed in pulpal axons. Scale bar = 20 µm.

Article Snippet: Preadsorption controls with blocking peptides for VGLUT1 (15 μg/ml; #135-3P, Synaptic Systems) and VGLUT2 (10 μg/ml; #G07B-VGLUT2-AG, Frontier Science) also completely abolished the respective staining.

Techniques:

Journal: PLoS ONE

Article Title: Expression of Vesicular Glutamate Transporters VGLUT1 and VGLUT2 in the Rat Dental Pulp and Trigeminal Ganglion following Inflammation

doi: 10.1371/journal.pone.0109723

Figure Lengend Snippet: Expression of VGLUT1 in pulpal axons in control (A) and CFA 1-day (B) and CFA 3-day (C) groups. VGLUT1 is expressed in many axons in the peripheral portion of the coronal pulp. D–I: Expression of VGLUT2 in pulpal axons in control (D, E), CFA 1-day (F), and CFA 3-day (G–I) groups. In the control group, VGLUT2 is expressed in a small number of axons in the peripheral portion of coronal pulp (D) and few axons in the radicular pulp (E). However, it is expressed in a large number of axons in the peripheral portion (F, G), the core of the coronal pulp (H), and in radicular pulp (I) in the CFA 1-day and CFA 3-day groups. Scale bar = 20 µm.

Article Snippet: Preadsorption controls with blocking peptides for VGLUT1 (15 μg/ml; #135-3P, Synaptic Systems) and VGLUT2 (10 μg/ml; #G07B-VGLUT2-AG, Frontier Science) also completely abolished the respective staining.

Techniques: Expressing, Control

Journal: PLoS ONE

Article Title: Expression of Vesicular Glutamate Transporters VGLUT1 and VGLUT2 in the Rat Dental Pulp and Trigeminal Ganglion following Inflammation

doi: 10.1371/journal.pone.0109723

Figure Lengend Snippet: VGLUT1 and VGLUT2 are expressed in many CD64+ cells (arrowhead) as well as in axons (arrow) in the inflamed dental pulp. Scale bar = 20 µm.

Article Snippet: Preadsorption controls with blocking peptides for VGLUT1 (15 μg/ml; #135-3P, Synaptic Systems) and VGLUT2 (10 μg/ml; #G07B-VGLUT2-AG, Frontier Science) also completely abolished the respective staining.

Techniques:

Journal: PLoS ONE

Article Title: Expression of Vesicular Glutamate Transporters VGLUT1 and VGLUT2 in the Rat Dental Pulp and Trigeminal Ganglion following Inflammation

doi: 10.1371/journal.pone.0109723

Figure Lengend Snippet: A : The density (area fraction) of VGLUT1+ pulpal axons is not significantly different between CFA 1-day or CFA 3-day groups and control, whereas the density of VGLUT2+ axons is significantly higher in the CFA 1-day, CFA 3-day groups than control. N = 3 animals in each group. *p<0.01. B : Representative images of the Western blot assay. C : Quantitative analysis of VGLUT1 and VGLUT2 protein in the dental pulp. Protein levels of VGLUT1 and VGLUT2 in the pulp are significantly higher in the CFA 1-day, CFA 3-day groups than control. This difference is bigger for VGLUT2 (3.9 and 4.3 fold higher in the CFA 1-day and CFA 3-day groups than control) than for VGLUT1 (2.9 and 3.1 fold higher in the CFA 1-day and CFA 3-day groups than control). N = 5 animals in each group. *p<0.01.

Article Snippet: Preadsorption controls with blocking peptides for VGLUT1 (15 μg/ml; #135-3P, Synaptic Systems) and VGLUT2 (10 μg/ml; #G07B-VGLUT2-AG, Frontier Science) also completely abolished the respective staining.

Techniques: Control, Western Blot

Journal: PLoS ONE

Article Title: Expression of Vesicular Glutamate Transporters VGLUT1 and VGLUT2 in the Rat Dental Pulp and Trigeminal Ganglion following Inflammation

doi: 10.1371/journal.pone.0109723

Figure Lengend Snippet: A: Immunofluorescent staining for VGLUT1 (a, b) and VGLUT2 (c, d) in the rat trigeminal ganglion in the control (a, c) and CFA 1-day (b, d) groups. VGLUT1 is expressed predominantly in medium- and large-sized somata (a, b), whereas VGLUT2 is expressed predominantly in small- and medium-sized somata (c, d). The number of VGLUT1+ somata of all somata is not different between control (a) and CFA 1-day group (b), whereas that of VGLUT2+ soma is significantly higher in the CFA 1-day (d) than in the control (c) groups. Scale bar = 50 µm. B: The density of VGLUT2+ somata (fraction of all somata) is significantly higher for the CFA 1-day, CFA 3-day groups than control, whereas the density of VGLUT1+ soma is not significantly different between CFA 1-day or CFA 3-day groups and control. N = 3 animals in each group. *p<0.01. C: Representative images of the Western blot assay for VGLUT1 and VGLUT2 in the rat trigeminal ganglion. D: Quantitative analysis of VGLUT1 and VGLUT2 protein in the trigeminal ganglion. The VGLUT2 protein levels are significantly higher in the CFA 1-day and CFA 3-day groups than for the control, whereas the VGLUT1 protein levels are not different between CFA 1-day or CFA 3-day groups and the control group. N = 5 animals in each group. *p<0.01.

Article Snippet: Preadsorption controls with blocking peptides for VGLUT1 (15 μg/ml; #135-3P, Synaptic Systems) and VGLUT2 (10 μg/ml; #G07B-VGLUT2-AG, Frontier Science) also completely abolished the respective staining.

Techniques: Staining, Control, Western Blot

Journal: Frontiers in Cellular Neuroscience

Article Title: Inhibition of GluR Current in Microvilli of Sensory Neurons via Na + -Microdomain Coupling Among GluR, HCN Channel, and Na + /K + Pump

doi: 10.3389/fncel.2018.00113

Figure Lengend Snippet: Glutamatergic axon terminals contact with HCN2-immunopositive small processes of the MTN neurons. (A , B) HCN2 immunoreactivity was localized in the small process and periphery of the MTN neuron. A terminal bouton (asterisk) contacted with an HCN2-immunopositive small process (arrowhead) of the MTN neuron (C) . (C) The white dotted line demarcates the border of the small process. HCN2 immunoreactivity was observable as an electron-dense immunoreactive product. (D) A VGLUT2-immunopositive axon terminal (gold particles, asterisk) contacted with an HCN2-immunopositive small process (arrowhead) of the soma of the MTN neuron. The white dotted line demarcates the border of the small process. (E , F) Terminal boutons (asterisks) containing round vesicles that were immunopositive for an anti-glutamate antibody (E) contacted with the processes of the MTN neurons. Note that the glutamatergic terminal (silver grains) formed asymmetrical synaptic contacts (arrowheads) with a process. The area enclosed by a rectangle in E is enlarged in F .

Article Snippet: Pre-adsorption with blocking peptides for VGLUT2 (15 mg/ml; #135-40P, Synaptic Systems) also completely abolished the respective staining.

Techniques: